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In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects
参考中译:加成型多层支架修复大面积骨软骨缺损的体内评价


          

刊名：Bio-Design and Manufacturing
作者：Tamaddon, Maryam（Univ Coll London）
Blunn, Gordon（Univ Portsmouth）
Tan, Rongwei（Shenzhen Lando Biomat Co Ltd）
Yang, Pan（Shenzhen Lando Biomat Co Ltd）
Sun, Xiaodan（Tsinghua Univ）
Chen, Shen-Mao（Univ Coll London）
Luo, Jiajun（Univ Coll London）
Liu, Ziyu（Univ Coll London）
Wang, Ling（Xi An Jiao Tong Univ）
Li, Dichen（Xi An Jiao Tong Univ）
Donate, Ricardo（Univ Las Palmas Gran Canaria）
Monzon, Mario（Univ Las Palmas Gran Canaria）
Liu, Chaozong（Univ Coll London）
刊号：780E0029
ISSN：2096-5524
出版年：2022
年卷期：2022, vol.5, no.3
页码：481-496
总页数：16
分类号：TH; Q81
关键词：Osteochondral scaffold；Large animal；Additive manufacturing；Porous titanium；COLLAGEN SCAFFOLDS；CARTILAGE REPAIR；PORE-SIZE；BONE；QUALITY；PLUG
参考中译：骨软骨支架；大动物；添加剂制造；多孔钛；胶原支架；软骨修复；孔径；骨；质量；塞子
语种：eng
文摘：The repair of osteochondral defects is one of the major clinical challenges in orthopaedics. Well-established osteochondral tissue engineering methods have shown promising results for the early treatment of small defects. However, less success has been achieved for the regeneration of large defects, which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the tissue. In this study, we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion techniques. The developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen "sandwich" composite system. The microstructure and mechanical properties of the scaffold were examined, and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle model. The 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen-HAp scaffold, and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage, as demonstrated by hyaline-like cartilage formation. The histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control group. Chondrogenic genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control group. The findings showed the safety and efficacy of the cell-free "translation-ready" osteochondral scaffold, which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects. [GRAPHICS] .
参考中译：骨软骨缺损的修复是骨科面临的主要临床挑战之一。成熟的骨软骨组织工程方法在早期治疗微小缺陷方面显示出良好的效果。然而，由于关节的机械环境和组织的异质性，在修复大的缺损方面取得的成功较少。在这项研究中，我们开发了一种多层骨软骨支架，通过利用添加剂制造技术并结合现有的激光烧结和材料挤压技术来匹配骨软骨组织的异质性。开发的支架是基于钛和聚乳酸基质增强的胶原“三明治”复合材料系统。对支架的微观结构和力学性能进行了检测，并在绵羊髁突模型上测试了其修复大面积骨软骨缺损的安全性和有效性。12周的体内评估期显示，与胶原-HAP支架相比，多层支架中广泛且明显更高的骨内生长，实现的稳定的机械固定为覆盖的软骨的愈合提供了强有力的支持，透明样软骨的形成证明了这一点。组织学检查显示，多层支架组再生的软骨优于对照组。成软骨基因聚集素和II型胶原在支架中表达上调，且高于对照组。研究结果显示了这种无细胞“平移准备”骨软骨支架的安全性和有效性，这种支架有可能用于治疗大型骨软骨缺损的一步手术过程中。[图形]。



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