【标题】In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects
【参考中译】加成型多层支架修复大面积骨软骨缺损的体内评价
【类型】 期刊
【关键词】 Osteochondral scaffold; Large animal; Additive manufacturing; Porous titanium; COLLAGEN SCAFFOLDS; CARTILAGE REPAIR; PORE-SIZE; BONE; QUALITY; PLUG
【参考中译】 骨软骨支架；大动物；添加剂制造；多孔钛；胶原支架；软骨修复；孔径；骨；质量；塞子
【作者】 Tamaddon, Maryam; Blunn, Gordon; Tan, Rongwei; Yang, Pan; Sun, Xiaodan; Chen, Shen-Mao; Luo, Jiajun; Liu, Ziyu; Wang, Ling; Li, Dichen; Donate, Ricardo; Monzon, Mario; Liu, Chaozong
【摘要】 The repair of osteochondral defects is one of the major clinical challenges in orthopaedics. Well-established osteochondral tissue engineering methods have shown promising results for the early treatment of small defects. However, less success has been achieved for the regeneration of large defects, which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the tissue. In this study, we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion techniques. The developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen "sandwich" composite system. The microstructure and mechanical properties of the scaffold were examined, and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle model. The 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen-HAp scaffold, and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage, as demonstrated by hyaline-like cartilage formation. The histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control group. Chondrogenic genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control group. The findings showed the safety and efficacy of the cell-free "translation-ready" osteochondral scaffold, which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects. [GRAPHICS] .
【参考中译】 骨软骨缺损的修复是骨科面临的主要临床挑战之一。成熟的骨软骨组织工程方法在早期治疗微小缺陷方面显示出良好的效果。然而，由于关节的机械环境和组织的异质性，在修复大的缺损方面取得的成功较少。在这项研究中，我们开发了一种多层骨软骨支架，通过利用添加剂制造技术并结合现有的激光烧结和材料挤压技术来匹配骨软骨组织的异质性。开发的支架是基于钛和聚乳酸基质增强的胶原“三明治”复合材料系统。对支架的微观结构和力学性能进行了检测，并在绵羊髁突模型上测试了其修复大面积骨软骨缺损的安全性和有效性。12周的体内评估期显示，与胶原-HAP支架相比，多层支架中广泛且明显更高的骨内生长，实现的稳定的机械固定为覆盖的软骨的愈合提供了强有力的支持，透明样软骨的形成证明了这一点。组织学检查显示，多层支架组再生的软骨优于对照组。成软骨基因聚集素和II型胶原在支架中表达上调，且高于对照组。研究结果显示了这种无细胞“平移准备”骨软骨支架的安全性和有效性，这种支架有可能用于治疗大型骨软骨缺损的一步手术过程中。[图形]。
【来源】 Bio-Design and Manufacturing 2022, vol.5, no.3
【入库时间】 2023/2/3
【标题】Application of additively manufactured 3D scaffolds for bone cancer treatment: a review
【参考中译】加成型三维支架在骨癌治疗中的应用
【关键词】 Additive manufacturing; Biomanufacturing; Bone cancer; Implants; Tissue engineering; DRUG-DELIVERY; EWINGS-SARCOMA; TUMOR-THERAPY; SPONTANEOUS REGRESSION; COMPOSITE SCAFFOLDS; PROGNOSTIC-FACTORS; RADIATION-THERAPY; LIMB SALVAGE; SOFT-TISSUE; SELENIUM
【参考中译】 添加剂制造；生物制造；骨癌；植入物；组织工程；药物输送；尤文斯肉瘤；肿瘤治疗；自发退化；复合支架；预后因素；放射治疗；保肢；软组织；硒
【作者】 Hou, Yanhao; Wang, Weiguang; Bartolo, Paulo
【摘要】 Bone cancer is a critical health problem on a global scale, and the associated huge clinical and economic burdens are still rising. Although many clinical approaches are currently used for bone cancer treatment, these methods usually affect the normal body functions and thus present significant limitations. Meanwhile, advanced materials and additive manufacturing have opened up promising avenues for the development of new strategies targeting both bone cancer treatment and post-treatment bone regeneration. This paper presents a comprehensive review of bone cancer and its current treatment methods, particularly focusing on a number of advanced strategies such as scaffolds based on advanced functional materials, drug-loaded scaffolds, and scaffolds for photothermal/magnetothermal therapy. Finally, the main research challenges and future perspectives are elaborated.
【参考中译】 骨癌在全球范围内是一个严重的健康问题，与之相关的巨大临床和经济负担仍在上升。尽管目前许多临床方法用于骨癌的治疗，但这些方法通常会影响正常的身体功能，因此存在很大的局限性。与此同时，先进材料和添加剂制造为开发针对骨癌治疗和治疗后骨再生的新策略开辟了有希望的途径。本文对骨肿瘤及其治疗方法进行了全面的综述，重点介绍了基于先进功能材料的支架、载药支架和光热/磁热治疗支架等先进策略。最后，阐述了本研究面临的主要挑战和未来展望。
【标题】Mapping of Bead Geometry in Wire Arc Additive Manufacturing Systems Using Passive Vision
【参考中译】基于被动视觉的弧焊添加剂制造系统中焊道几何形状的映射
【关键词】 Wire arc additive manufacturing; Passive vision; Bead geometry measurement; WAAM characteristic monitoring; SEAM TRACKING; TECHNOLOGIES; WIDTH
【参考中译】 焊丝电弧添加制造；被动视觉；焊道几何测量；WAAM特性监测；焊缝跟踪；技术；宽度
【作者】 Couto, Marcus O.; Rodrigues, Arthur G.; Coutinho, Fernando; Costa, Ramon R.; Leite, Antonio C.; Lizarralde, Fernando; Payao Filho, Joao C.
【摘要】 This work addresses the online extraction of the geometry characteristics (width and centerline) of deposited beads with monocular cameras for wire arc additive manufacturing (WAAM). To enable online measurement and feature extraction from captured images, an adaptive threshold is used for segmentation, a Canny algorithm for edge detection, a Hough-line transform for feature identification of the bead edges, and a filtering step to attenuate the low signal-to-noise ratios of deposition processes. Online measurements are performed in single-bead and layer (multi-bead) scenarios. The proposed vision-based solution is experimentally implemented in a WAAM robotic system composed of a welding torch, a Kuka KR90 robot arm, a power source, wire feeder, and a passive monocular camera. Experimental results illustrate the performance and effectiveness of the proposed visual-based methodology.
【参考中译】 本文研究了利用单目摄像机在线提取焊丝弧焊过程中熔珠的几何特征(宽度和中心线)。为了能够从捕获的图像中进行在线测量和特征提取，使用自适应阈值进行分割，使用Canny算法进行边缘检测，使用Hough-line变换进行珠子边缘的特征识别，并使用滤波步骤来衰减沉积过程的低信噪比。在线测量是在单珠和层(多珠)情况下进行的。提出的基于视觉的解决方案在由焊枪、库卡KR90机械臂、电源、送丝机和被动单目摄像机组成的WAAM机器人系统中进行了实验实现。实验结果表明了基于视觉的方法的性能和有效性。
【来源】 Journal of control, automation and electrical systems 2022, vol.33, no.4